Chemotactic and Haptotactic Properties of Mesothelioma

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One fascinating study is called, "Chemotaxis and Haptotaxis of Human Malignant Mesothelioma Cells: Effects of Fibronectin, Laminin, Kind IV Collagen, and an Autocrine Motility Factor-like Substance" by Julius Klominek, Karl-Henrik Robért, and Karl-Gösta Sundqvist - Cancer Res September 15, 1993 53 4376.  Here is an excerpt: "Abstract - A human malignant pleural mesothelioma cell line (STAV) was studied with respect to production of the extracellular matrix components laminin, kind IV collagen, and fibronectin, and interactions with these proteins in vitro. We also analyzed STAV cell serum-zero cost conditioned medium with respect to the attainable presence of "autocrine motility factor-like" substance. Sodium dodecylsulfate-polyacrylamide gel electrophoresis of biosynthetically labeled STAV serum-cost-free conditioned medium showed that STAV cells released a variety of proteins into the medium, such as components with molecular weights of 850,000, 540,000 and 440,000. Using Western blotting we identified these proteins as laminin, type IV collagen, and fibronectin, respectively. By immunocytochemistry laminin, kind IV collagen, and fibronectin had been detected as a matrix surrounding the cells. Plastic culture dishes coated with µg quantities of laminin, kind IV collagen, and fibronectin induced attachment and spreading of STAV cells. Laminin, type IV collagen, and fibronectin stimulated directional (chemotactic) migration of STAV cells in Boyden chambers fitted with 8 µm filters. The exact same cells also migrated to insoluble step gradients of filter-bound extracellular matrix components (haptotaxis).

When STAV serum-free of charge conditioned medium was separated by working with quickly protein liquid chromatography Superose 6 gel filtration, two motility-inducing protein peaks were detected. The initial peak contained proteins with molecular weight > 220,000 that had each chemotactic and haptotactic properties, whilst the second peak contained material with apparent molecular weights of roughly 67,000 that had chemotactic and chemokinetic (random motility) but not haptotactic properties. Analysis of the Mr 67,000 material indicated that it was a heat-sensitive and trypsin-digestible protein. The production of both soluble and insoluble extracellular matrix components by human mesothelioma cells and the motile response to these molecules as properly as the production of a Mr 67,000 autocrine motility factor-like substance could possibly be imperative for the highly invasive motile behavior of this tumor."  Another intriguing study is known as, "Complementary value of 5 carcinoma markers for the diagnosis of malignant mesothelioma, adenocarcinoma metastasis, and reactive mesothelium in serous effusions" by Mick Delahaye C.T. (I.A.C.), Frieda van der Ham, Theo H. van der Kwast M.D., Ph.D. - Diagnostic Cytopathology Volume 17, Problem two, pages 115–120, August 1997.  Here is an excerpt: "Abstract - Cytological slides of serous fluids of 41 malignant mesotheliomas, 88 metastatic adenocarcinomas, and 25 reactive effusions were immunostained with the antibodies anti-CEA, MOC-31, Leu-M1, Ber-EP4, and B72.three. Most mesotheliomas and all reactive fluids failed to stain with these antibodies. The sensitivity of the 5 markers to detect carcinoma cells differed remarkably. Particularly MOC-31, Ber-EP4, and B72.3 stained with a high quantity of carcinoma circumstances and the complementary value of Ber-EP4 and B72.three to immunostain carcinoma cells was impressive: 94% of the metastatic adenocarcinoma circumstances reacted with Ber-EP4 or B72.3 in contrast to 1 of 41 malignant mesotheliomas." Diagn. Cytopathol. 17:115–120, 1997